activation with pha Search Results


94
Chem Impex International carboxyphenol ba
Carboxyphenol Ba, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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InvivoGen pha-p
Pha P, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Difco pha activation (phytohemagglutinin-p
Pha Activation (Phytohemagglutinin P, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza pha-activated pbmc cells
A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & <t>D)</t> <t>PHA-activated</t> <t>PBMC</t> (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.
Pha Activated Pbmc Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pha-activated pbmc cells - by Bioz Stars, 2026-03
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90
BioMimetic Therapeutics pet-g-pha active packaging films
A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & <t>D)</t> <t>PHA-activated</t> <t>PBMC</t> (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.
Pet G Pha Active Packaging Films, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioMimetic Therapeutics iron chelation by biomimetic pet-g-pha active packaging films
A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & <t>D)</t> <t>PHA-activated</t> <t>PBMC</t> (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.
Iron Chelation By Biomimetic Pet G Pha Active Packaging Films, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Boehringer Mannheim pha-activated head kidney cdna library in pcdna 3.1
A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & <t>D)</t> <t>PHA-activated</t> <t>PBMC</t> (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.
Pha Activated Head Kidney Cdna Library In Pcdna 3.1, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pha-activated head kidney cdna library in pcdna 3.1/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
pha-activated head kidney cdna library in pcdna 3.1 - by Bioz Stars, 2026-03
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A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & D) PHA-activated PBMC (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.

Journal: Virology

Article Title: Use of ATP analogs to inhibit HIV-1 transcription

doi: 10.1016/j.virol.2012.06.007

Figure Lengend Snippet: A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & D) PHA-activated PBMC (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.

Article Snippet: E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, MTT Assay, Software, Infection, Trypan Blue Exclusion Assay