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Image Search Results
Journal: Virology
Article Title: Use of ATP analogs to inhibit HIV-1 transcription
doi: 10.1016/j.virol.2012.06.007
Figure Lengend Snippet: A) CYC202, CR8 and CR8#13 were used to treat activated OM10.1 cells. Cells were grown to mid-log phase of growth and treated with TNF-α for 2 hours to induce HIV-1 expression. TNF-α was then removed and cells were subsequently treated with drugs at 0.001, 0.01, 0.1, 1, 10 and 100 µM in complete media. Five days later, sups were collected and processed for p24 HIV-1 Gag ELISA. B) At day five cells were processed for MTT assay. Analysis was carried out with Prizm software using non-liner regression curve-fit of sigmoidal dose-response with variable slope. C & D) PHA-activated PBMC (5 × 106) were infected with THA/92/00 strain (MOI: 0.1). After 8 hours, unadsorbed virus was washed away and cells were treated with 10 or 100 nM of either Flavopiridol or CR8#13. Supernatants were collected every 3 days for RT analysis. Number of live cells was also plotted from each date and treatment (trypan blue exclusion assay). E &F) Similar to panel C, where PHA-activated PBMC cells (Lonza) were infected with the THA/92/00 strain and treated with various concentrations of Flavopiridol or CR8#13 cells. Samples were assayed using CellTiter-Glo after either 3 or 16 days.
Article Snippet: E &F) Similar to panel C, where PHA-activated
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, MTT Assay, Software, Infection, Trypan Blue Exclusion Assay